Rudimentary construction of liver histioids by hyaluronic acid scaffold
- VernacularTitle:透明质酸支架构建工程化肝组织小块的初步研究
- Author:
Yingxin XU
;
Wenjie WANG
;
Yong ZHANG
;
Shihe WU
;
Xuhua SONG
;
Juchao LIU
;
Rong LI
;
Qingling FENG
- Publication Type:Journal Article
- Keywords:
liver, tissue engineering, hyaluronic acid (HA), implantation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(7):550-551
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the possibility of the liver histioid construction through co-culturing hepatocytes and sinusoidal vessel endothelial cells (SVECs) on hyaluronic acid(HA) scaffold.MethodsHepatocytes and SVECs of a mouse were isolated respectively, and then were cultured successively in HA scaffold. The scaffold-cell complexes formed later were implanted onto the surfaces of liver in the mouse. After 2 weeks, the implants were taken out for HE staining and compatibility evaluation.ResultsThe isolated hepatocytes and SVECs were vigorous. The implants inosculated well with liver tissue of the host with visible growth of blood vessels in the implants. The hepatocyte aggregation grown around the vessels, the liver-like tissue, were observed.ConclusionIt is feasible to construct liver histioid through co-culturing hepatocytes and sinusoidal vessel endothelial cells on hyaluronic acid scaffold.
