Protective effect of pyrroloquinoline quinone on damage of hippocampal neurons induced by NMDA in rats
- VernacularTitle:吡咯喹啉醌对NMDA诱发大鼠海马神经元损伤的影响
- Author:
Shunhua XIONG
;
Guixiang FENG
;
Lian LI
;
Xingwen WANG
- Publication Type:Journal Article
- Keywords:
pyrroloquinoline quinone (PQQ), hippocampal neurons, N-methyl-D-aspartate(NMDA), rat
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(7):509-511
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the protective effect of pyrroloquinoline quinone (PQQ) on damage of hippocampal neurons induced by NMDA.MethodsHippocampal neurons were cultured in vitro and NMDA was used to induce neurotoxicity 8 d after, while PQQ were used or not before. The metamorphosis of hippocampal neurons was observed under the microscope. Intracellular calcium levels was measured by confocal laser microscopy. ResultsThe intracellular Ca2+ level increased rapidly after exposure to 0.1 mmol/L NMDA and resulted in neuronal necrosis and apoptosis. Otherwise, pretreatment of the cultured neurons with PQQ reduced the increase of the intracellular Ca2+ level and the neuronal necrosis or apoptosis induced by NMDA.ConclusionPQQ can protect hippocampal neurons from damage induced by NMDA.
