Effect of β-adrenergic receptor stimulation on vascular smooth muscle cell apoptosis
- VernacularTitle:血管平滑肌β-肾上腺能受体对细胞凋亡的影响
- Author:
Jing MA
;
Ming ZHENG
;
Shi-wen WANG
;
Yusheng ZHAO
- Publication Type:Journal Article
- Keywords:
β-adrenergic receptor, vascular smooth muscle cell, apoptosis
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(5):271-273
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of β-adrenergic receptor(β2AR) stimulation on vascular smooth muscle cell apoptosis in physiological state and receptor overexpression model.Methodsβ2AR overexpression model was established by transgenic techniques. Hoechst 33342 staining as well as flow cytometer(FCM) detected were chosen to measure the incidence of vascular smooth muscle cell apoptosis.ResultsVascular smooth muscle cell exhibited significantly fewer viable cell rate when stimulated with β2AR agonist isoproteronol for 48 hours compared with control (P<0.01),while no difference at the time point of 24 hours. Much fewer viable rate detected by FCM and high apoptotic rate by Hoechst staining were observed when VSMCs overexpressing β2AR were stimulated with isoproteronol for 24 hours (P<0.01).ConclusionStimulation of physiological and overexpressing β2AR could induce apoptosis of vascular smooth muscle cell.
