Effect of Ningshen Ling and dehydroepiandrosterone on the cognitive function in mice subjected to chronic mild stress
- VernacularTitle:宁神灵冲剂和脱氢表雄酮对慢性轻度应激小鼠认知功能的影响
- Author:
Nan YANG
;
Rui-feng GAO
;
Ping-Ping ZUO
- Publication Type:Journal Article
- Keywords:
chronic mild stress (CMS), Ningshen Ling (Chinese herbs), dehydroepiandrosterone (DHEA), central cholinergic M receptor, spontaneous movement, Morris water maze, mice
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(5):268-270
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the alteration of behavior,such as spontaneous movement,spatial memory ability and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS). And to determine whether Ningshen Ling (NSL)and dehydroepiandrosterone(DHEA)could reverse the cognitive impairment in this model.MethodsForty mice were divided into four groups: control group,CMS group,CMS+NSL(p.o.5g·kg-1·d-1) group and CMS+DHEA (i.p.5 mg·kg-1·d-1) group. Morris water maze procedure was used to determine the spatial memory ability. M receptor binding activity was measured with radioactivity assay by3 H-QNB.ResultsAfter 3 weeks CMS,the mice exerted a decrease in spontaneous movement test,and the latency in Morris water maze was obviously prolonged. Treating CMS mice with NSL and DHEA for 1 weeks could improve the spontaneous movement and latency declined. Whereas the3 H-QNB binding ability to M receptor showed a significantly decrease in cerebral cortex and hippocampus of CMS mice (P<0.05),the decreased ability of M receptor binding was reversed by NSL treatment in hippocampus(P<0.05).ConclusionNSL and DHEA can alleviate the stress response and reversed cognitive impairment induced by CMS,and it may be concerned with the central cholinergic M receptors activity.
