Pro-culture with serum promoting neural stem cell proliferation
- VernacularTitle:血清预培养促进神经干细胞的增殖
- Author:
Junhua LI
;
Shurong ZHENG
;
Hong WAN
;
Shaodong ZHANG
- Publication Type:Journal Article
- Keywords:
neural stem cell, pre-culture, serum, proliferation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(5):339-340
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation.
