Effect of Earle's solution and MEM on activities of neurons in culture

VernacularTitle:Earle's液与MEM培养基对神经元活性的影响
Author: Ming HAN ; Liping DONG ; Fang YUAN
Publication Type:Journal Article
Keywords: neurons, Earle's solution, minimal essential medium (MEM), lactate dehydrogenase (LDH), 3-(4, 5-dimethylthiazol)2, 5-diphenyltetrazolium bromide (MTT)
From: Chinese Journal of Rehabilitation Theory and Practice 2005;11(2):125-126
CountryChina
Language:Chinese
Abstract: ObjectiveTo observe the activities of cultured neurons incubated with Earle's solution, new minimal essential medium (MEM) or former MEM.MethodsActivity changes of cultured neurons were measured by determining the leakage of lactate dehydrogenase (LDH) and metabolic rate of 3-(4,5-dimethylthiazol)2,5 diphenyltetrazolium bromide (MTT).ResultsActivities of neurons incubated with different culture medium for 12 h or 24 h were significantly different (P<0.01).ConclusionDifferent culture medium can influence neuronal activities.