Necrosis and apoptosis induced by brucea javanica oil emulsion in bladder cancer cell
- VernacularTitle:鸦胆子油乳对膀胱癌细胞系BIU-87坏死与凋亡的影响
- Author:
Xiao-wu LI
;
He WANG
;
Wei-jun QIN
;
Xin LI
;
Tao LIU
;
Chunmei FAN
- Publication Type:Journal Article
- Keywords:
brucea javanica, bladder neoplasm, necrosis, apoptosis, mitochondria, cytochrome C
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(3):163-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effcet of necrosis and apoptosis induced by Brucea javanica oil emulsion on bladder cancer BIU-87 cells. MethodsBrucea javanica oil emulsion of different concentration was added to the bladder cancer BIU-87 cell cultured in vitro. The ratio of apoptosis/necrosis cells and mitochondrial membrane potential(MMP) were detected with flow cytometer. The dissipation of cytochrome C was observed with confocal microscopy. ResultsHigh concentration brucea javanica oil emulsion reduced MMP which led cell necrosis, while that of low concentration diffused the cytochrome C from mitochondria to cytoplasm which induced cell apoptosis. ConclusionHigh concentration brucea javanica oil emulsion mainly causes BIU-87 cells to necrosis, low concentration induces cell to apoptosis.
