Effect of bone marrow mesenchymal stem on the deep partial thickness derma burn injury in guinea pigs
- VernacularTitle:骨髓间充质干细胞移植对豚鼠皮肤深Ⅱ度烧伤的治疗作用
- Author:
Huhetana BAO
;
Li GUO
;
Ling LING
;
Rifeng LU
;
Jianhui WANG
;
Fei YIN
;
Hongxue FAN
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells, burn, cell transplantation, therapy, guinea pigs
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(1):13-15
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of bone marrow mesenchymal stem cells transplantation on the deep partial thickness derma burn injury in guinea pigs.MethodsSuspension cells of MSCs were cultured in vitro and transplanted into wounds of the female guinea pig of the deep partial thickness derma burn injuring model with the concentration of 2×106cell/ml(group A),2×107cell/ ml(group B). The rate of wound healing was observed, factor Ⅷ was detected using immunohistochemistry. Y chromosome on the wound in guinea pig was detected using PCR 15,30,50 days after MSCs had been transplanted. ResultsThe rate of wound healing of groups A and B was better than that in the control group, but there was not significantly different between groups A and B. Microvessel density of wound in groups A and B was better than those in the control group on the 15th and 30th days. There was not significantly different between the group A and B. PCR results showed that Y chromosome gene was expressed on some recoveried wound of female guinea pig. ConclusionBone marrow mesenchymal stem cells transplantation is effective in deep partial thickness derma burn injury in guinea pigs.
