Effect of transplantation of the neural stem cells on the cholinergic neurons in hippocampus of vascular dementia rats
- VernacularTitle:移植神经干细胞对血管性痴呆大鼠海马胆碱能神经元的影响
- Author:
Jianxin YE
;
Wei WANG
;
Zhi ZHENG
- Publication Type:Journal Article
- Keywords:
neural stem cells, transplantation, vascular dementia, hippocampus, cholinergic neurons, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the change of cholinergic neurons of hippocampus of vascular dementia rats after transplantation of the neural stem cells(NSCs) in hippocampus.MethodsAfter the vascular dementia model was reproduced,the rats were randomly divided into transplantation,dementia and pseudo-operative groups.8 weeks after operation,immunofluorescence was used to observe the survivors and migration of neural stem cells.Immunochemistry staining was used to observe the number of ChAT positive neurons in hippocampus.ResultsThe number of the ChAT positive neurons in the CA1 subfield of the hippocampus were more significantly increased in transplantation group than that in dementia group. ConclusionChAT positive neurons can be found in hippocampus area of vascular dementia rats after transplanting NSCs into the hippocampus, which seem to be cholinergic neurons.
