Nude mouse as in vivo bioreactor: The feasibility study of construction transplantable tissue engineered bone
- VernacularTitle:以裸鼠为活体生物反应器构建可移植组织工程骨的可行性研究
- Author:
Qing XI
;
Rongfa BU
;
Hongchen LIU
- Publication Type:Journal Article
- Keywords:
bone mesenchymal stem cell, bone tissue engineering, immunological reaction, bone transplantation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(1):7-9
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the feasibility of construction transplantable tissue engineered bone in nude mouse as in vivo bioreactor. MethodsSelected the 4th passage of rabbit bone mesenchymal stem cells, which were cultured, expanded and induced in vitro. Cells were seeded into porous natural coral. The complexes were implanted subcutaneous in nude mice. Regenerated bone was taken out 8 weeks after implantation and implanted into muscle tissue interspace in the back of rabbit itself.The rabbit-anti-mouse antibody titer was measured at 14,28,56 days and the level of IL-2 was detected the at 2, 7, 14, 28 and 56 days after implantation.Immunohistochemistry staining was carried on in the 8th week. ResultsDuring the whole experimental process, IL-2 activity couldn't be measured.The rabbit-anti-mouse antibody titer was positive.A specific antibody reaction was detected from 2ed week to 8th week after transplantation and touches the peak value four weeks later.Immunohistochemistry staining result was negative.ConclusionThe tissue engineered bone growth in nude mouse is seemed to be the reliable transplantable bone source.
