Osteoinductivity of recombinent human bone morphogenetic protein-2 gene mediated with cationic liposome transfer in skeletal muscle stem cells of rabbits in vitro
- VernacularTitle:阳离子脂质体介导重组人骨形态发生蛋白-2基因转染的兔骨骼肌干细胞体外成骨分化的实验研究
- Author:
Yong SHANG
;
Ji WU
;
Shibi LU
;
Mei YUAN
- Publication Type:Journal Article
- Keywords:
bone morphogenetic protein, cationic liposome, skeletal muscle stem cells, gene therapy, bone defect, rabbit
- From:
Chinese Journal of Rehabilitation Theory and Practice
2005;11(1):1-3
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the osteoinductivity of cationic liposome mediated recombined human bone morphogenetic protein-2 gene (rhBMP-2) transfer in skeletal muscle stem cells (SMSCs) of rabbits. MethodsSMSCs were transfered by pAGFP rhBMP-2 liposome complex. Gene transferred SMSCs were evaluated with immunohistochemistry.ResultsThe transfection rate of SMSCs transferred by cationic liposome mediated pAGFP-rhBMP-2 was 14.18%. Positive stain of rhBMP-2, alkaline phosphatase(ALP),collegenⅠhad been found in the transferred SMSCs.ConclusionQuantity of SMSCs is enough to be target cells of gene therapy. Cationic liposome can mediated bone morphogenetic protein-2 gene transfected SMSCs in vitro. Compared with AdrhBMP-2,the transfection rate of liposome mediated bone morphogenetic protein-2 gene transfected SMSCs is lower.
