Optimization of the preparation process of Soft-shelled turtle blood lyophilized powder using Box-Behnken response surface methodology
- VernacularTitle:Box-Behnken响应面实验优化鳖血冻干粉的制备工艺
- Author:
Yue LOU
1
;
Xuerong SU
1
;
Chunqin MAO
1
;
Xiaoli ZHAO
1
;
Tulin LU
1
,
2
;
Wenxia PI
1
Author Information
1. College of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Traditional Chinese Medicine Processing and Inheritance Base，National Administration of Traditional Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
soft-shelled turtle blood;
vacuum freeze-drying;
solubility;
process optimization
- From:
China Pharmacy
2023;34(13):1573-1576
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the preparation process of Soft-shelled turtle blood lyophilized powder （STBLP）， and to provide a reference for improving the availability and quality stability of soft-shelled turtle blood （STB）. METHODS STBLP was prepared with vacuum freeze-drying. Taking the solubility as the index， the preparation process parameters of STBLP were optimized by single factor experiment and Box-Behnken response surface method. RESULTS The optimal freeze-drying process for STBLP was obtained： pre-freezing time of 4 h， total drying time of 13 h （before at 0 ℃）， and resolution drying temperature of 25 ℃. The average solubility of 3 batches of STBLP prepared according to the optimal process was 95.72% （RSD＝0.68%， n＝3）， the relative error of which was －0.97% to the theoretical solubility （96.66%）. CONCLUSIONS Optimized lyophilization process in this study are stable and feasible， the solubility of the prepared sample is high.
