Effect of dendritic cells sensitized by tumor cells lysates on the murine breast cancer
- VernacularTitle:肿瘤细胞裂解物致敏树突状细胞对小鼠乳腺癌作用的研究
- Author:
Xin JIA
;
Rong LI
;
Ying-xin XU
;
Shaoyou XIA
;
Li LI
- Publication Type:Journal Article
- Keywords:
dendritic cell, breast carcinoma, immunotherapy
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(2):79-81
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of dendritic cells (DC) sensitized by tumor cells lysates on the murine breast cancer.MethodsBone marrow-generated DCs were sensitized by EMT6 tumor cell lysates, and tested whether they produced antigens to T lymphcytes and induced tumor-specific CTL. Antigen-sensitized DCs were also used to treat the mice with breast carcinoma.ResultsDCs sensitized by tumor cell lysates markedly led to the proliferation of lymphcytes in vitro, and induced tumor specific CTL that significantly difference compared with PBS control group (P=0.027). Meanwhile, DCs sensitized by tumor lysates could inhibit the growth of established murine breast cancer that also significantly difference compared with PBS control group (P=0.035).ConclusionDCs sensitized by tumor cell can induce an effective antitumor immune response both in vitro and in vivo.
