Morroniside Inhibits H_2O_2-Induced Oxidative Injury in SH-SY5Y Neuroblastoma Cells

VernacularTitle:莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞氧化损伤
Author: Houxi AI ; Wenting HUANG ; Wen WANG ; Lin LI ; Yi AN
Publication Type:Journal Article
Keywords: Morroniside, neurocyte, oxidative stress, nitric oxide, glutathione
From: Chinese Journal of Rehabilitation Theory and Practice 2007;13(11):1023-1025
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of Morroniside on H2O2-induced oxidative injury in SH-SY5Y neuroblastoma cells.MethodsSH-SY5Y cells were pre-incubated with Morroniside(1,10,and 100 μmol/L)for 24 h prior to exposure to H2O2(500 μmol/L)for 18 h.The content of reactive oxygen species(ROS),nitric oxide(NO),glutathione(GSH)were determined.ResultsPretreatment the cells with Morroniside(10 and 100 μmol/L)lowered H2O2-induced ROS release by 37%(P<0.01)and 27%(P<0.05)versus H2O2,and NO release in a dose-dependent manner by 31%(P<0.05),53%(P<0.01)versus H2O2.Morroniside(1,10,and 100 μmol/L)increased GSH level in cells treated with H2O2 in comparison with cells exposed only to H2O2,respectively.ConclusionMorroniside shows neuroprotection effect against H2O2-induced oxidation injury in SH-SY5Y cell.