A new method for isolation of neural stem cells
- VernacularTitle:一种获得神经干细胞单细胞的新方法
- Author:
Jia-mei LIU
;
dong CHEN
;
Xiao-ting MENG
- Publication Type:Journal Article
- Keywords:
neural stem cells(NSCs), Dispase, dissociation
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(1):19-20
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate an effective method to isolate neural stem cells(NSCs).MethodsNSCs were dissociated by digestion with trypsin, EDTA and different doses of Dispase,and serum-free culture techniques and immunohistochemistry techniques were used to verifying the dissociated.ResultsA lot of single neural stem cells were obtained by using Dispase to digest neurosphere, and the cells could keep its structure and morphology.ConclusionIt is an ideal method by using Dispase to digest neurosphere for isolating NSCs.
