Expression of specific proteins of neural cell in amniotic epithelial cells in rats
- VernacularTitle:神经组织细胞特异性蛋白在大鼠羊膜上皮细胞中的表达
- Author:
Xiao-ting MENG
;
Dong CHEN
;
Jia-mei LIU
;
Laijin LU
- Publication Type:Journal Article
- Keywords:
neural cells, amniotic epithelial cells(AECs), immunocytochemistry, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(1):17-18
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect specific antigens of neural cells in amniotic epithelial cells(AECs) in rats. MethodsAECs were dissociated and purified from the amnion of pregnancy 12—14 d rats. The expression of specific markers of neural stem cells (Nestin, Musashi) and differentiated cells (MAP-2,NSE,GFAP) and ChAT, NT-3 in the AECs were detected by immunocytochemistry. ResultsThe cultured AECs displayed positive immunoreactivity to MAP-2, NSE, GFAP, Nestin and Musashi. In addition, the cells also demonstrated immunoreactivity to ChAT and NT-3. ConclusionAECs are similar with neural cells and it may be useful as a sustained source to improve outcome of neural stem cells transplantation.
