Observation of the morphological change during the adipogenesis differentiation of human bone marrow stromal cell
- VernacularTitle:人骨髓基质干细胞体外诱导成脂过程形态学变化的观察
- Author:
Lin ZHAO
;
Zhi-ming YANG
;
Li DENG
;
Huiqi XIE
;
Xiuqun LI
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cell, induction, differentiation, adipogenesis, morphology
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(1):15-16
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the variant differentiated phase of the human bone marrow stromal cell (MSC) during induced adipogenesis course by morphological observation.MethodsAfter proliferated in vitro, MSCs isolated from bone marrow of the female adults volunteers were cultured with the adipogenetic inducers for 6—30 days. Morphological changes of the cells were observed everyday under Olympus contrast phase microscopy, and some specimens were stained with Oil-red-O.ResultsMSC showed long spindle shape before inducing, and then changed gradually to oval or round shape and the figure enlarged simultaneously. There were specifically morphological changes with lipid droplet emergence under plasmalemma and confluence gradually to lipid drop during differentiated into the phase of immature and mature adipocyte.ConclusionIt is easy to detect the MSC differentiated into the phase of immature and mature adipocyte with the specific morphological change of lipid droplet, while in the phase of preadipocyte and adipoblast, there is no special morphological change, and it may need some specific markers to detect.
