Mechanism of differentiation of mesenchymal stem cells into neuron-like cells in vitro
- VernacularTitle:骨髓间充质干细胞体外诱导分化为神经样细胞的机制
- Author:
Qian CHU
;
Ya-ping WANG
;
Xin-qiao FU
;
Suming ZHANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells(MSCs), differentiation, neuron-like cells, neural stem cells(NSCs)
- From:
Chinese Journal of Rehabilitation Theory and Practice
2004;10(1):13-14
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the mechanism of differentiation of mesenchymal stem cells(MSCs) into neuron-like cells in vitro.MethodsMSCs of Wistar rats were separated and cultured, and then induced with DMSO and BHA in vitro. The specific marking proteins of neurons, glia and neural stem cells were detected before preinduction, at 24h after preinduction, at 6h, 24h, and 48h after neuronal induction.ResultsAfter the inducement, many MSCs turned into bipolar,multipolar and taper,and then intersected as network structure. Nestin was strong positive at 6h after neuronal induction, and decreased at 24h, 48h after the induction. NeuN was present at 6 h after neuronal induction, and increased at 24h, 48h after the induction.ConclusionMSCs can be induced into neural stem cells(NSCs) at first, and then differentiate into neuron-like cells in vitro.
