Isolation and identification of multidrug-resistant Escherichia coli from cattle, sheep, poultry and human in Cumilla, Bangladesh

Md Abul Fazal; Chandan Nath; Md Sirazul Islam; F M Yasir Hasib; Md Moktadir Billah Reza; Himadri Shankar Devnath; Md Nahid-ibn-rahman; Abdul Ahad

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Isolation and identification of multidrug-resistant Escherichia coli from cattle, sheep, poultry and human in Cumilla, Bangladesh

Author: Md Abul Fazal ¹ ; Chandan Nath ¹ ; Md Sirazul Islam ² ; F M Yasir Hasib ^{3
,

4} ; Md Moktadir Billah Reza ⁵ ; Himadri Shankar Devnath ⁶ ; Md Nahid-Ibn-Rahman ⁷ ; Abdul Ahad ¹
Author Information

1. Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram-4225, Bangladesh.
2. Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram-4225, Bangladesh.
3. Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram-4225, Bangladesh. &
4. Department of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong SAR.
5. Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram-4225, Bangladesh.
6. Ministry of Health and Family Planning, Peoples Republic of Bangladesh.
7. Department of Animal Science and Nutrition, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram-4225, Bangladesh.
Publication Type:Other Types
Keywords: Commensal E. coli; Multidrug-resistant; Resistance gene; Animal; Human
MeSH: Escherichia coli; Drug Resistance, Multiple, Bacterial
From:Malaysian Journal of Microbiology 2022;18(2):227-234
CountryMalaysia
Language:English
Abstract: Aims:Antimicrobial resistance (AMR) is a significant public health concern of modern civilization. The potential risk of AMR is significant in terms of both human and animal health. This study aims to assess the antimicrobial resistance pattern of selected antimicrobials against Escherichia coli of animal, poultry and human origin in the Cumilla district of Bangladesh.
Methodology and results:A total of 200 samples were collected from different sources. Isolation and identification of commensal E. coli were performed following standard bacteriological and molecular techniques. Antimicrobial susceptibility testing was performed following the Kirby-Bauer disc diffusion technique. Ampicillin, tetracycline and sulfamethoxazole-trimethoprim resistance genes were detected by polymerase chain reactions (PCR). A total of 152 (76%; 95% confidence interval (CI) 70-81%) E. coli were isolated from cattle, sheep, chicken and human, where 37.5% of isolates were found to be multidrug-resistant (MDR). In the cultural sensitivity test, E. coli showed the highest resistance to sulfamethoxazole-trimethoprim (71%), tetracycline (63%), ampicillin (62%), where gentamicin (23%) showed the lowest resistance, followed by ceftriaxone (26%). The prevalence of resistance genes like blaTEM, tetA, tetB, tetC, sul1 and sul2 were 100%, 95%, 11%, 8%, 58% and 52%, respectively.
Conclusion, significance and impact of study:The emergence of multidrug-resistant commensal E. coli and resistance genes circulating in animals, poultry and humans limit the treatment options for serious infections.
Full text:20.2022my00231.pdf