Comparison of nutrient-rich and limited media in the production of biosurfactant by Achromobacter xylosoxidans BP(1)5
- Author:
Silvia Kurnia Sari
1
,
2
;
Ni’matuzahroh
1
,
3
,
4
;
Fatimah
1
,
2
;
Tri Nurhariyati
1
,
2
;
Nastiti Trikurniadewi
1
,
2
;
Ana Mariatul Khiftiyah
1
,
2
;
Achmad Zainal Abidin
1
,
2
;
Khudrotun Nisa Indriyasari
5
Author Information
- Publication Type:Journal Article
- Keywords: Achromobacter xylosoxidans; Biosurfactant; Agricultural and industrial wastes; Green product
- MeSH: Achromobacter denitrificans; Surface-Active Agents
- From:Malaysian Journal of Microbiology 2022;18(2):215-221
- CountryMalaysia
- Language:English
-
Abstract:
Aims:This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media.
Methodology and results:This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m.
Conclusion, significance and impact of study:The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium. The use of rice straw will contribute to the reduction of biosurfactant production costs and valorisation of agricultural waste. - Full text:20.2022my0021.pdf
