Expression and Significance of Differential Expression Gene Hepsin in the Prostate Cancer
- VernacularTitle:差异表达基因Hepsin在前列腺癌中的表达及其意义
- Author:
Wen-li LIANG
;
Li-qun ZHOU
;
Dian-qi XIN
;
Yanqun NA
- Publication Type:Journal Article
- Keywords:
prostate cancer, gene expression, Hepsin gene
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(12):1060-1062
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression and significance of differential expression gene Hepsin in the prostate cancer (PC) screened by the cDNA microarray technique.Methods The techniques of semiquantitative RT-PCR and Western blotting were used to detect the mRNA and protein expression of Hepsin. Specimens of 40 cases of PC, 15 benign prostatic hyperplasia (BPH) and 6 normal prostate tissues were examined by the immunohistochemical stain.Results Hepsin was more expressed in PC tissue than normal prostate tissue (P=0.026) and was confirmed by Western blot analysis. Immunohistochemical test confirmed this and demonstrated that Hepsin did not expressed in normal prostate but expressed in PC and BPH and there was a significant difference in Hepsin expression level between PC and BPH tissues ( P=0.000).Conclusion Hepsin high expressed in PC may be a new molecular marker in early diagnosis of PC and a new target for gene therapy of PC.
