Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatocyte-like Cells Induced by Rebirth Liver Tissue Lixivium of Mouse in Vitro
- VernacularTitle:小鼠再生肝浸出液体外诱导骨髓间充质干细胞向肝细胞样分化的研究
- Author:
Jun-lan YU
;
Rong LI
;
Wen-jie WANG
;
Li LI
;
Juchao LIU
;
Jinjing WANG
;
Yingxin XU
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells (BMSCs), hepatocyte-like cells, differentiation, tissue engineering
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(12):1056-1057
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce bone marrow mesenchymal stem cells (BMSCs) differentiated into hepatocyte-like cells with rebirth liver tissue lixivium of mouse in vitro.Methods Mouse BMSCs were isolated and directionally induced with rebirth liver tissue lixivium of mouse 36 h after partial hepatectomy (PH). The morphology of cells was observed under an invert microscope, and the characteristics of differentiated cells was identified by immunofluorescence test.Results 7 days after induced by liver lixivium, the spindle shaped BMSCs became round and resembled hepatocyte-like cells. 1～2 weeks later, the differentiated cells expressed albumin, CK8 and CK18, which were known as characteristic markers of the hepatocyte.Conclusion BMSCs of mouse can be differentiated into hepatocyte-like cells under induction of liver tissue lixivium of mouse.
