Expression of Bone Morphogenetic Protein-2 in Gunshot Fracture Healing Detected with Real Time Fluorescence Quantitative Reverse Transcript Polymerase Chain Reaction
- VernacularTitle:实时荧光定量逆转录聚合酶链反应检测火器伤骨折愈合过程中骨形态发生蛋白的表达
- Author:
Bing-sheng LI
;
Ying-jian CHEN
;
Qiang ZHANG
;
Changchun FAN
;
Jibo ZHENG
;
Jinfang CAI
- Publication Type:Journal Article
- Keywords:
gunshot fracture, bone morphogenetic protein-2(BMP-2), real time fluorescence quantity, reverse transcription polymerase chain reaction(RT-PCR)
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(11):963-965
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect the levels of bone morphogenetic protein-2(BMP-2) mRNA in gunshot fracture healing of the rabbits.MethodsA model of primary treating gunshot fracture of the rabbit with external fixator and the real time fluorescence quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR) technology with SYBR Green I were established.The levels of BMP-2 mRNA 1,2,3 and 4 weeks after operation were detected with FQ-RT-PCR respectively.ResultsIn the control group,BMP-2 mRNA of the bone tissue began to rise at the first week after fracture and the peak of mRNA expression appeared at the second week.The expression returned to normal at the forth week.In the experimental group,the BMP-2 mRNA rose more slowly that it arrived peak stage at the third week and was significantly lower in experimental group than that in control group at the same phases.ConclusionReal time FQ-RT-PCR is a good method for measuring the levels of BMP-2 mRNA during gunshot fracture healing.The mRNA expression rose more slowly and was significantly lower in gunshot fracture than in common fracture at the same phases.
