The potential use of papaya and banana peels as substrate to enhance the pigment production of Gram-positive bacterial strain isolated from Holothuria (Lessonothuria) pardalis

Siti Najihah Solehin; Kamarul Rahim Kamarudin; Nur Sabrina Badrulhisham; Aisyah Mohamed Rehan

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The potential use of papaya and banana peels as substrate to enhance the pigment production of Gram-positive bacterial strain isolated from Holothuria (Lessonothuria) pardalis

Author: Siti Najihah Solehin ¹ ; Kamarul Rahim Kamarudin ¹ ; Nur Sabrina Badrulhisham ¹ ; Aisyah Mohamed Rehan ²
Author Information

1. Centre of Research for Sustainable Uses of Natural Resources (SUNR), Faculty of Applied Sciences and Technology (FAST), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Campus, Pagoh Education Hub, KM 1, Jalan Panchor, 84600 Muar, Johor Darul Tazim, Malaysia.
2. Department of Chemical Engineering Technology, Faculty of Engineering Technology (FTK), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Campus, Pagoh Education Hub, KM 1, Jalan Panchor, 84600 Muar, Johor Darul Tazim, Malaysia.
Publication Type:Journal Article
Keywords: Fruit waste substrate; Pigment; Gram-positive bacteria; Optimization; Stability test
MeSH: Gram-Positive Bacterial Infections; Holothuria; Carica; Musa
From:Malaysian Journal of Microbiology 2022;18(2):136-145
CountryMalaysia
Language:English
Abstract: Aims:The microbial pigment can be the best promising alternative to replace synthetic colorant. However, due to the high cost of synthetic medium for microbial pigment production, there is a need to develop a new low-cost medium of bacterial pigment production. This study aims to investigate the potential of banana and papaya peels as alternative lowcost substrates for a carotenoid-producing bacterium, B12 strain (bacteria strain isolated from Holothuria (Lessonothuria) pardalis).
Methodology and results:B12 strain identified as an aerobic bacterium with non-motile, diplobacilli shaped and Grampositive bacteria. The fermentation was optimized with different parameters included the effect of temperature, time, concentrations, pHs, carbon and nitrogen sources to find the optimum relative pigment concentration produced by B12. The results showed that the B12 strain produced the highest relative pigment concentration measured at 450 nm when the strain was cultivated at 37 °C and pH 7 in the culture medium incorporated with the combination of dried papaya peels and banana peels (100% v/v with ratio 1:1) at 72 h of incubation. Lactose, peptone and yeast were observed as the best carbon and nitrogen sources to increase the pigment concentration of B12 strain. Stability of the pigment was studied at different physiochemical stress, and it showed the pigment obtained from dried papaya and banana substrates can tolerate and stable under stress condition.
Conclusion, significance and impact of study:This can be concluded that the combination of dried papaya and banana peels worked well as substrate and can be utilized as a fermentation medium to replace the synthetic medium which is more expensive and uneconomical for industry application. Besides, it also helps in managing waste and solving the pollution problem due to the increasing of biochemical oxygen demand (BOD) and chemical oxygen demand (COD).
Full text:20.2022my0014.pdf