Antimicrobial and physicochemical characterization of Lactobacillus brevis biofilms as biopreservative agents

Amalia Solichah; Fadilla Sapalina; Endah Retnaningrum

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Antimicrobial and physicochemical characterization of Lactobacillus brevis biofilms as biopreservative agents

Author: Amalia Solichah ¹ ; Fadilla Sapalina ^{2
,

3} ; Endah Retnaningrum ⁴
Author Information

1. Graduate Student of the Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara Yogyakarta, 55281, Indonesia.
2. Graduate Student of the Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara Yogyakarta, 55281, Indonesia. &
3. Indonesian Oil Palm Research Institute, Jl. Brigjend Katamso No. 51, Medan, Sumatera Utara, 20158, Indonesia.
4. Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara Yogyakarta, 5528, Indonesia.
Publication Type:Journal Article
Keywords: Foodborne disease; Pathogenic bacteria; Adhesion; SEM-EDS; Rod-shaped
MeSH: Levilactobacillus brevis; Lactobacillales; Foodborne Diseases
From:Malaysian Journal of Microbiology 2022;18(1):93-104
CountryMalaysia
Language:English
Abstract: Aims:Lactic acid bacteria (LAB) biofilms constitute one of the most remarkable breakthroughs in the field of food biopreservatives and can be employed to prevent foodborne disease. The purposes of this study were to investigate the efficacy of inhibitory LAB biofilms against foodborne pathogens and evaluate their tolerance to acidic pH and bile salts, as well as their physicochemical properties.
Methodology and results:Four strains of Lactobacillus brevis biofilms isolated from kimchi showed antipathogenic activity to the bacteria Staphylococcus aureus FNCC 0049 and Escherichia coli FNCC 0091. These biofilms were also tolerant to pH 2.5, 0.3% bile salt and strong adhesion. Two of the four L. brevis biofilms (L. brevis biofilm KA2 and KB1) produced the highest inhibitory activity against both pathogenic bacterial indicators, tolerance to acidic pH and bile salts, and the strongest adhesion. In addition, based on Scanning Electron Microscope-Energy Dispersion X-ray Spectroscopy (SEM-EDS) analysis, both biofilm strains had a smooth surface texture; the cell morphology was rod-shaped and consisted of several elements such as carbon, oxygen and nitrogen, which was built up of extracellular polymeric substances (EPS).
Conclusion, significance and impact of study:The presence of EPS as a constituent of LAB biofilms influenced their survival abilities in an acidic pH and bile salt environment. As a result, the characteristics of L. brevis biofilm KA2 and KB1 made them excellent candidates for use as antimicrobial packaging systems in food biopreservative applications.
Full text:20.2022my0009.pdf