Novel pan-lineage VP1 specific degenerate primers for precise genetic characterization of serotype O foot and mouth disease virus circulating in India
- Author:
Sagar Ashok KHULAPE
1
;
Jitendra Kumar BISWAL
;
Chandrakanta JANA
;
Saravanan SUBRAMANIAM
;
Rabindra Prasad SINGH
Author Information
- Publication Type:Rapid Communication
- From:Journal of Veterinary Science 2023;24(3):e40-
- CountryRepublic of Korea
- Language:English
- Abstract: Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.
