The anti-inflammatory effects of angiotensin II blockade in animal model of cyclosporine-induced nephropathy.
- Author:
Joon Chang SONG
1
;
Mi Jung SHIN
;
Yeon Joo JUN
;
Seong Yong WOO
;
Bok Jin HYOUNG
;
Hye Eun YOON
;
Hyun Young WOO
;
Beum Soon CHOI
;
Chul Woo YANG
;
Yong Soo KIM
;
Yoon Sik CHANG
;
Byung Kee BANG
Author Information
1. Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea. yangch@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Cyclosporine nephropathy;
Angiotensin II blockade;
C-reactive protein;
Osteopontin;
ED-1
- MeSH:
Angiotensin II*;
Angiotensins*;
Animals*;
C-Reactive Protein;
Creatinine;
Diet, Sodium-Restricted;
Fibrosis;
Humans;
Inflammation;
Kidney;
Losartan;
Male;
Models, Animal*;
Olea;
Osteopontin;
Rats;
Rats, Sprague-Dawley;
Renin-Angiotensin System;
RNA, Messenger;
Olive Oil
- From:Korean Journal of Medicine
2004;67(6):615-624
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Long-term treatment of immunosuppresant CsA causes interstitial inflammation and fibrosis in the kidney. Renin-angiotensin system (RAS) plays the most important role in the pathogenesis CsA-induced renal injury. Accordingly we evaluated the anti-inflammatory effect of angiotensin II blockades using losartan (LSRT) in a rat model of chronic CsA nephropathy. METHODS: Male Sprague-Dawley rats, initially weighing 225 to 250 g, were used. After 1 week of a low-salt diet (0.05% sodium), the rats were randomized into four groups and treated for 4 weeks. The Vehicle (VH) group was treated with olive oil. The VH+LSRT group was treated with olive oil and LSRT. The CsA group received CsA. The CsA+LSRT group was simultaneously treated with CsA and LSRT. The anti-inflammatory effect of LSRT was evaluated with C-reactive protein (CRP) expression, osteopontin (OPN) mRNA and protein expression, and ED-1 infiltration RESULTS: The CsA treatment caused an increase in serum creatinine and a decrease in creatinine clearance compared with that of the VH group. Intrarenal CRP positive cells were significantly decreased in the CsA+LSRT group compared with the CsA group (38.0 +/- 2.1 vs. 65.0 +/- 5.1, p<0.01). In the CsA group, the degree of OPN mRNA expression was increased compared with that of the VH group. But, OPN mRNA expression was decreased in the CsA+LSRT group (387.5 +/- 56.6% vs. 719.8 +/- 58.5%, p<0.05). In the degree of ED-1 infiltration, we had a similar results such as CRP and OPN mRNA expression (CsA group 30.5 +/- 8.0 vs. CsA+LSRT 86.0 +/- 11.0, p<0.01). CONCLUSION: We concluded that the anti-inflammatory effects of angiotensin II blockade has a potential protective effect against CsA-induced renal injury.
