Species differences of Baishouwu based on characteristic chromatogram and content determination of acetophenones
10.12206/j.issn.2097-2024.202207036
- VernacularTitle:基于特征图谱及苯乙酮类成分含量测定的白首乌种属差异研究
- Author:
Meiyu LIN
1
;
Wenhua CHEN
2
;
Lingchuan XU
2
;
Ting HAN
1
;
Juan SU
1
;
Chi ZHANG
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
- Publication Type:Originalarticles
- Keywords:
Baishouwu;
acetophenone;
HPLC;
characteristic chromatogram;
contents determination
- From:
Journal of Pharmaceutical Practice
2023;41(6):366-371
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To provide scientific basis for the quality control of different species of Baishouwu by establishing the HPLC fingerprint of domestic of Baishouwu and determining the main active components of acetophenones. Methods HPLC-DAD method was used to determine the HPLC fingerprints of domestic of Baishouwu. Then, the content of 4 kinds of acetophenones in Baishouwu was determined. The column was Diamonsil C18（250mm×4.6mm, 5μm）with the mobile phase of methanol and 0.1% phosphoric acid at a flow rate of 1.0 ml/min. The detection wavelength of p-Hydroxyl acetophenone, baishouwu benzophenone, 2',4'-Dihydroxy acetophenone was set at 260 nm and 2',5'-Dihydroxy acetophenone at 280 nm respectively. Results The similarity and cluster analysis in HPLC fingerprint showed that the constituents were significantly different among C. bungei, C. auriculatum and C. wilfordii. The content of total acetophenones in C. wilfordii was significantly higher than that in other localities of C. auriculatum and C. bungei. Conclusions Acetophenone could be used as the evaluation index to evaluate the quality of Baishouwu in different origins. The content of total acetophenone in C. wilfordii is the highest, which could be used as the best quality resource of Baishouwu.
