In vivo pharmacokinetic study on determination of effective components in Xiakucao Xiaoliu mixture in Normal Rat Plasma By LC-MS/MS
10.12206/j.issn.2097-2024.202111008
- VernacularTitle:LC -MS/MS法测定大鼠血浆中夏枯草消瘤合剂有效成分体内药动学研究
- Author:
Yuanyuan DAI
1
,
2
;
Minghua MA
3
;
Xiaohong XU
4
;
Xiaohe WANG
4
;
Ruoxi ZHANG
5
;
Zhiru XU
6
;
Hua NIAN
5
Author Information
1. Yueyang hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Tradional Chinese Medcine, Shanghai 200437 China
2. Shanghai International Medical Center, Shanghai 200120 China.
3. Department of Pharmacy, Yangpu Hospital, Tongji University, Shanghai 200090 China.
4. The Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 201306 China.
5. Yueyang hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Tradional Chinese Medcine, Shanghai 200437 China.
6. State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203 China.
- Publication Type:Originalarticles
- Keywords:
Pharmacokinetics;
Xiakucao Xiaoliu mixture;
LC-MS
- From:
Journal of Pharmaceutical Practice
2023;41(6):358-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method and study the pharmacokinetics for concentration determination of effective components in Xiakucao Xiaoliu mixture in Normal Rat Plasma By LC-MS/MS. Methods The mobile phase was methanol-water (0.1% formic acid) system under the positive ion mode of C18 chromatographic column, gradient elution was adopted, and the flow rate was 0.3 ml/min. In the negative ion mode, the mobile phase was acetonitrile-water (0.1% formic acid) system, gradient elution, with a flow rate of 0.4 ml/min. Caffeic acid, rosmarinic acid, syringic acid, rutin in positive ion mode and Atractylodes lactone II and Atractylodes lactone III in negative ion mode were respectively determined. Normal rats were intragastrically given Xiakucao Xiaoliu Mixture 7.8 ml/kg, and blood was taken from the orbit at different time points after the administration. The blood concentration was determined by the validated LC-MS/MS method and the non-standard DAS2.0 software was used. The pharmacokinetic parameters of rats after administration were calculated by the compartment model. Results The pharmacokinetic parameters belonged to non atrioventricular model. The pharmacokinetic characteristics of the four main anti-cancer active ingredients of Caffeic acid, Rosmarinic acid, Syringic acid and Atractylodes Ⅲ in rats after administration of Xiakucao Xiaoliu Mixture were significantly different from those reported in the literature after the administration of monomers. Conclusion The established method was simple, accurate and sensitive, which could be suitable for the content determination of effective components in Xiakucao Xiaoliu mixture in Normal Rat Plasma, which would be a valuable information for the study on main anticancer active substances.
