Effect of Reduced Glutathione on Expression of Malondialdehyde,Glutathione Peroxidase and Superoxide Dismutase after Focal Cerebral Infarction in Rats
- VernacularTitle:还原型谷胱甘肽对大鼠局灶性脑梗死后丙二醛、谷胱甘肽过氧化物酶、超氧化物歧化酶表达的影响
- Author:
Ya-qin LU
;
Yan-feng SONG
;
Yi-ping HOU
- Publication Type:Journal Article
- Keywords:
reduced glutathione(GSH), malondialdehyde(MDA), glutathione peroxidase(GSH-PX), superoxide dismutase(SOD), focal cerebral infarction, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(11):923-925
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of reduced glutathione(GSH) on expression of malondialdehyde(MDA),glutathione peroxidase(GSH-PX) and superoxide dismutase(SOD) after focal cerebral infarction in rats.MethodsRat models of middle cerebral artery occlusion(MCAO) were estabilished with thread after 2-hour ischemia and 6-hour reperfusion.Rats were divided at random into three groups,i.e.,sham-operated,control and treatment group(with GSH 1200 mg/kg) respectively.After the rats model was performed,neurology deficit score,the size of brain infarct region and the change of brain tissue pathologic were evaluated.Contents of MDA and activity of SOD and GSH-PX were detected with spectrophotometer.ResultsCompared with the control group,GSH can ameliorate neurological deficit score and decrease infarct volume induced by MCAO.GSH may reduce contents of MDA and improve activity of SOD and GSH-PX in brain tissue.ConclusionGSH may reduce contents of MDA and improve activity of SOD and GSH-PX so as to enhance capability of eliminating oxygen free radical,and play a neuroprotective effect after cerebral focal ischemia reperfusion.
