Study results of the composition of oral microflora in Mongolian patients with Behcet’s disease
- VernacularTitle:Бехчетийн өвчинтэй монгол өвчтөнүүдийн амны хөндийн микрофлорын бүрэлдхүүнийг судалсан дүн
- Author:
Javzandulam B
1
,
2
;
Osamu U
3
;
Nobuyoshi K
4
,
5
;
Baasankhuu J
1
;
Sarantuya J
6
Author Information
1. Department of Ophthalmology, School of MNUMS
2. Glaucoma Clinic, Zalaa Khukh Tolgoi LLC
3. Health Sciences University of Hokkaido School of Dentistry
4. Department of Ophthalmology, Health Sciences University of Hokkaido
5. Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University
6. Department of Molecular Biology and Genetics, School of Bio-Medicine, Mongolian National University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Behcet's disease;
16S rRNA;
S24-7;
Akkermansia
- From:Innovation
2021;14(1-Ophthalmology):42-45
- CountryMongolia
- Language:Mongolian
-
Abstract:
Purpose:Behcet’s disease is characterized by repeated acute inflammatory attacks with
aphthous ulcers of the oral mucosa, uveitis of the eyes, skin symptoms, and genital ulcers. Although
its etiology is still unknown, there is evidence of the involvement of oral bacteria in systemic
diseases. Various types of oral bacteria may be involved in the development and progression of
Behcet's disease. Therefore, the present study investigated alterations in the oral flora of patients
with Behcet’s disease in Mongolia. We collected saliva samples from the Mongolian Behcet's
disease (BD) group and healthy control (HC) group, and the oral flora were analyzed using next generation sequencer (NGS).
Methods:DNA was extracted from the unstimulated saliva samples from the 47 BD and 48 HC
subjects. The DNA was amplified from the V3-V4 region of 16S rRNA using PCR, and the data were
acquired using NGS. Based on the obtained data, we analyzed the alpha diversity, beta diversity,
and bacterial taxonomy of the salivary flora.
Household survey covered 148 people with visual and hearing impairments to assess social service
accessibility.
Results:Beta diversity differed significantly between the BD and HC flora, but no significant
differences were observed in alpha diversity. We found that the proportions of three genera—an
S24-7 family unknown species, a mitochondria family unknown species, and Akkermansia species were significantly lower in the BD than in the HC group.
Conclusion:The reduced proportions of the S24-7 family and symbiotic Akkermansia species
may be key phenomena in the oral flora of patients with BD.
- Full text:InnoOpht-2021-14(1);42-45.pdf
