VEGF-A and ARMS2 gene polymorphism on AMD susceptibility
- VernacularTitle:Насжилт хамааралт шар толбоны сөнөрөл ба зарим генүүдийн хамаарал
- Author:
Ariunzaya A
1
,
2
;
Chimedlkhamsuren G
3
;
Suvd T
3
;
Sarantuya J
3
;
Uranchimeg D
1
;
Baasankhuu J
2
Author Information
1. MNUMS, Ophthalmology department
2. Bolor melmii Eye Hospital
3. MNUMS, School of Biomedicine, Molecular biology-Genetic department
- Publication Type:Journal Article
- Keywords:
allele frequency;
age related macular degeneration;
meta-analysis;
single nucleotide polymorphism
- From:Innovation
2019;13(1-Ophthalmology):10-13
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Age-related macular degeneration (AMD) is an eye condition, that occurs
people aged above 50, leads to gradual loss of the vision because of a damage in the macula,
which is located in the center of the retina. Several polymorphisms in different genes have been
proposed as factors that increase the disease susceptibility. Therefore, we investigated the
association between rs833061 polymorphism of VEGF-A gene and rs10490924 polymorphism of
ARMS2 gene and AMD in order to analyze with other similar studies by meta analysis.
Purpose:To investigate the polymorphisms of VEGF-A gene and ARMS2 gene on AMD susceptibility
Methods:is case-control study was conducted on 74 AMD patients and 32 unaffected age-and gender-matched control individuals. Genomic DNA was extracted from the peripheral
venous blood. The single nucleotide polymorphisms were identified by restriction fragment length
polymorphism (RFLP) method and results confirmed by gel electrophoresis. The REVIEW MANAGER
5.2 software and MetaXL was used for meta-analysis
Results:We did not find statistically significant differences in С allele and СС genotype frequency
of rs833061 polymorphism of VEGF-A gene between patients and controls. However, analysis of
rs10490924 polymorphism of ARMS2 gene shows that T allele (OR=2.72, 95% CI, 1.47 – 5.02, p=0.001),
TT genotype (OR=4.54, 95% CI, 1.49 – 13.87,p=0.019) were significantly associated with AMD risk.
Haplotype analysis of these SNPs showed that C+T haplotype was statistically significantly different
(OR=5.23, 95% CI, 1.76-15.54, p=0.002) between patients and controls.
Conclusion:As shown by results, rs10490924 polymorphism of ARMS2 gene show that T allele,
TT genotype and C+T haplotype were significantly associated with AMD risk In meta-analysis, T
allele of rs10490924 polymorphism of ARMS2 gene was significantly associated with AMD risk in all
ethnicity that include Asian and Caucasian. However, T allele prevalence was higher in Asians.
- Full text:InnoOpht-2019-13(1);10-13.pdf
