Influence of rs16924159 polymorphism of IL-33 gene on asthma susceptibility
- VernacularTitle:IL-33 генийн rs16924159 полиморфизм гуурсан хоолойн багтраа өвчинд хүргэх өртөмхий байдалд нөлөөлөх нь
- Author:
Chimedlkhamsuren G
1
;
Uyangakhorol N
1
;
Batbold O
2
,
3
;
Ichinnorov D
4
;
Sarantuya J
1
Author Information
1. Department of Molecul biology-Genetic, Bio-Medicine School, Mongolian National University of Medical Science
2. Department of Pulmonology-Allergology, School of Medicine, Mongolian National University of Medical Science
3. Kor Hospital
4. Department of Pulmonology-Allergology, School of Medicine, Mongolian National University of Medical Science
- Publication Type:Journal Article
- From:Innovation
2020;14(2):67-71
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Interleukin-33 (IL-33) cytokine plays a crucial role in asthma pathogenesis. Recent
studies have established that IL-33 activity was increased in serum, airway smooth muscle and
epithelial cells from patients with asthma and this increase positively correlates with asthma
severity. We hypothesized that several genetic variations that contributing IL-33 expression
and activity, which may risk factor for susceptibility to asthma. In this study, we examined the
association between rs16924159 single nucleotide polymorphism (SNP) of IL-33 gene and asthma
susceptibility.
Methods:51 asthma patients and 54 healthy volunteers were involved in this case-control
study. Blood sample was collected for genomic DNA extraction. rs16924159 SNP genotyping was
performed by the allele specific-polymerase chain reaction (AS-PCR) method. Statistical analysis
was performed using STATA 13.0 software.
Results:The groups were matched for age, gender and body mass index (p>0.05). The distribution
of rs16924159 allele and genotypes among patients and controls was found in accordance with
those expected by the Hardy-Weinberg equilibrium (p=0.088). Adenine (A) allele frequency of
rs16924159 was significantly different between case and control groups (OR = 1.91, 95% CI = 1.04-
3.51, p = 0.037). Also, homozygote A/A (OR=6.53, 95% CI 0.68-62.38, p=0.104) and heterozygote
(OR=2.08, 95% CI 0.93-4.62, p=0.073) genotypes were more frequent among asthma patients than
in controls.
Conclusions:From these findings, we conclude the A allele of rs16924159 SNP in IL-33 gene
may be contributing to asthma susceptibility, increasing the carrier`s risk to the development of
asthma.
- Full text:Innovation-2020-14(2);67-71.pdf
