Effect and mechanism of matrine on steatosis Chang Liver cells induced by oleic acid
- VernacularTitle:苦参碱对油酸诱导的脂肪变性ChangLiver细胞的影响及机制
- Author:
Limei YANG
1
;
Jie ZHUANG
1
;
Fenyan CHEN
1
;
Xuhui HUANG
1
Author Information
1. Dept. of Pharmacy，Fujian Provincial Hospital/ Provincial Clinical Medical College of Fujian Medical University，Fuzhou 350001，China
- Publication Type:Journal Article
- Keywords:
matrine;
Chang Liver cells;
lipid metabolism;
nonalcoholic fatty liver disease
- From:
China Pharmacy
2023;34(12):1456-1459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of matrine （MT） on steatosis Chang Liver cell model induced by oleic acid （OA） and its possible mechanism. METHODS Chang Liver cells were divided into blank group， model group and MT low-dose， medium-dose group and high-dose groups （0.1， 0.5， 1.0 mmol/L）. Except for blank group， the other groups were treated with 1.0 mmol/L OA for 24 h to establish steatosis model， and MT groups were given corresponding concentrations of drugs for 24 h. The activities of steatosis Chang Liver cells were observed； the morphologies of intracellular lipid droplets were observed and lipid content was also determined. The contents of liver function indexes [alanine transaminase （ALT）， aspartate transaminase （AST）， total bilirubin （TBIL）， alkaline phosphatase （ALP）]， as well as mRNA and protein expressions of farnesoid X receptor （FXR）， cytochrome P450 7A1 （CYP7A1） and fibroblast growth factor 19 （FGF19） were all detected. RESULTS OA and MT had no significant effect on the activity of Chang Liver cells. After OA treatment， orange lipid droplets formed in cytoplasm； compared with blank group， relative lipid content and the levels of liver function indexes were increased significantly， while the mRNA and protein expressions of FXR， CYP7A1 and FGF19 were down-regulated significantly （P＜0.05）. After treatment of low， medium and high concentrations of MT， above indexes were all reversed significantly （P＜0.05）. CONCLUSIONS MT could significantly improve the lipid content and liver function indexes of steatosis Chang Liver cells induced by OA though regulating FXR/CYP7A1/ FGF19 signaling pathway.
