Genome sequencing and analysis of BCG strain for vaccine production in China
10.19428/j.cnki.sjpm.2023.22427
- VernacularTitle:卡介苗生产用菌株全基因组测序
- Author:
Zhaoyang LIU
1
;
Yongqiang ZHU
2
;
Yingmei XU
1
;
Yueye ZHAO
1
;
Tiaoxia ZHU
1
;
Leijun MA
1
;
Zhewen CHEN
1
Author Information
1. Shanghai Institute of Biological Products Co., Ltd., Shanghai 201403, China
2. Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 201203, China
- Publication Type:Journal Article
- Keywords:
BCG D2PB302 strain;
IVE⁃TB antigen;
MTBVAC;
whole genome sequencing
- From:
Shanghai Journal of Preventive Medicine
2023;35(3):213-218
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo conduct the sequencing and preliminarily analysis of the whole genome of BCG Shanghai D2PB302 strain （hereinafter referred to as BCG Shanghai D2 strain）， which has been used exclusively for the vaccine production in China. MethodsThe DNA of of BCG Shanghai D2 strain （D2-JIA12-1） was extracted， and the whole genome was sequenced by Pacbio-RS Ⅱ. The sequence data was assembled by Smrtlink and polished with the illumina data. Genes， tRNA and rRNA were predicted based on the sequence data. The functional annotation of predicted genes was performed through BLASTP. The IVE-TB antigen gene and MTBVAC were selected as the target sequences to be compared with Mycobacterium tuberculosis H37Rv （NC_000962.3）. ResultsThe sequence length of BCG Shanghai D2 strain was 4 045 232 bp， and the GC content was 65.66%. A total of 4 259 protein-encoding genes were predicted， with an average gene size of 933 bp. 2 476 genes had biological functions and others were hypothetical proteins.144 virulence genes were obtained by comparing with the VFDB. There were 29 type Ⅶ secretion system genes and 10 PE/PPE protein family genes. ConclusionThe whole genome sequence of BCG Shanghai D2 strain is clarified. It lays a broad foundation for subsequent detection of the stability of major antigen genes.
