Inducing Mesenchymal Stem Cells from Adult Human Adipose Tissue to Differentiate into Cardiomyocytes In Vitro
- VernacularTitle:成人脂肪间充质干细胞体外诱导分化为心肌细胞的实验研究
- Author:
Jing XIA
;
Guang-hui CHEN
;
Hong-bin LIU
;
Li WANG
- Publication Type:Journal Article
- Keywords:
adipose tissue-derived mesenchymal stem cells, cardiomyocyte, differentiation, induce in vitro
- From:
Chinese Journal of Rehabilitation Theory and Practice
2006;12(9):783-784
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the differentiation of adipose tissue-derived mesenchymal stem cells (ADMSCs) into cardiomyocytes in vitro. MethodsADMSCs were isolated and purified by the method of digesting and adhering to the culture plastis. The third generation of cells were determined with immunocytochemistry method and induced by 5-aza. On the 7th, 14th, 21st, 28th day after being induced, the surface antigen of myoblast cell and cardiac myocyte were determined, the expression of gene of GATA4 and Nkx2.5 were tested, and the content of atrial natriuretic polypeptide (ANP) assayed. ResultsImmunocytochemical staining showed CD44, CD13, CD105 positive, CD45, CD34, HLA-DR, factor Ⅷ negative. After being induced by 5-aza, the direction of cell arraying was gradually similar. The cells were stained positively for Desmin, α-Sarcomeric Actin, myosin heavy chain and Troponin T. The cells express GATA4 and Nkx2.5 and secrete ANP. ConclusionThere was mesenchymal stem cells in human adipose tissue. 5-aza may induce ADMSCs to differentiate into cardiomyocytes in vitro.
