Mechanism of BCL2L2-PABPN1 expression induced by sodium arsenite and its metabolites in 16HBE cells
- Author:
SHI Ya YIN Jin yao WU Jiang JIANG Cheng lan ZHAO Rui huan ZHOU Qian HE Yue feng
- Publication Type:Journal Article
- Keywords:
Sodium arsenite Fusion gene BCL 2 like protein 2 BCL2L2 Poly A binding protein nuclear 1 PABPN1 BCL2L2-PABPN1； ； ； Apoptosis P53 Signal pathway
- From:
China Occupational Medicine
2022;49(05):522-
- CountryChina
- Language:Chinese
-
Abstract:
Objective - - （BCL2L2）- （ ）
To investigate the differential expression of the fusion gene BCL 2 like protein 2 poly A
（PABPN1） （ ）
binding protein nuclear 1 induced by sodium arsenite SA and its methylated metabolites in 16HBE cells and the
Methods ） ，
related mechanism. i The 16HBE cells exposed to SA at concentrations of 1.5 3.0 and 4.5 µmol/L were set as
-， - -
low medium and high dose arsenic exposure groups. The 16HBE cells exposed to 4.5 µmol/L monomethylarsonic acid
（ ）， （ ） ，
MMA dimethylarsonic acid DMA and SA were set as MMA group DMA group and SA group. The 16HBE cells without
， BCL2L2-PABPN1
toxic stimulation were set as control group. After the cells were cultured for 48 hours the expression of was
- （ - ） ） （ ）
detected by quantitative real time polymerase chain reaction qRT PCR . ii Two small interfering RNA siRNA silencing
基金项目：国家自然科学基金（ ）； 年云南省科技厅昆明医科大学应用基础研究联合专项面上项目
82160607 2021
（ ）
202101AY070001-054
作者简介：施雅（ —），女，在读大学本科生，主要从事劳动卫生与环境卫生学研究；尹锦瑶（ —），女，在读劳动卫生与环境卫
2001 1995
生学硕士研究生，主要从事劳动卫生与环境卫生学研究；施雅和尹锦瑶为共同第一作者
通讯作者：何越峰教授，博士研究生导师，- ：
E mail heyuefeng@kmmu.edu.cn中国职业医学 年 月第 卷第 期 ， ， ， · ·
2022 10 49 5 Chin Occup Med October 2022 Vol.49 No.5 523
BCL2L2-PABPN1， -
fragments were designed and transfected into 16HBE cells to knockdown which were set as siRNA 1 group
- - BCL2L2-PABPN1
and siRNA 2 group. Non transfected control group without knockdown of transfection was set up. After
， BCL2L2-PABPN1 -
culturing for 48 hours the expression level of in the three groups of cells was detected by qRT PCR. The cell
-
survival rate and early apoptosis rate were detected by MTS method and JC 1 mitochondrial membrane potential detection
， （ ） ，
method respectively. The apoptosis was detected by Hoechest33342/propidium iodide PI double staining and the expression
- Results ）
level of P53 signaling pathway related proteins was detected by Western blotting. i The relative expression of
BCL2L2-PABPN1 （P ） BCL2L2-
in 16HBE cells increased with the increasing SA doses <0.01 . The relative expression of
PABPN1 - ， - -
in high dose arsenic exposure was higher than that in control group low dose and medium dose arsenic exposure
（ P ） BCL2L2-PABPN1 ，
groups all <0.05 . The relative expression of in SA group was higher than those in control group MMA
（ P ） BCL2L2-PABPN1
group and DMA group all <0.05 . The relative expression of showed no significant difference between
， （ P ） ） BCL2L2-PABPN1
control group MMA group and DMA group all >0.05 . ii The relative expression levels of and cell
- - - （ P ）
survival rate in siRNA 1 group and siRNA 2 group were lower than those in non transfected control group all <0.05 .
， （P ）
However there was no significant difference in the early apoptosis rate among the three groups >0.05 . The results of
-
Hoechest33342/PI double staining showed that the number of nuclear shrinkage and early apoptotic cells in siRNA 1 group and
- - ， -
siRNA 2 group was higher than that in non transfected control group. The relative protein expression levels of P53 phospho
， - - ， - - （ P ）
p53 BCL 2 associated death promoter P21 and cytochrome C in siRNA 1 group and siRNA 2 group were higher all <0.05 ,
- - P
and the relative protein expression levels of P53 up regulated modulator of apoptosis were lower (all <0.05), when compared
- Conclusion
with the non transfected control group. SA may block the apoptosis of 16HBE cells by inducing the expression of
BCL2L2-PABPN1
fusion gene . The mechanism may be related to the activation of P53 signaling pathway. The SA methylated
BCL2L2-PABPN1 BCL2L2-PABPN1 -
metabolites MMD and DMA had no effect on the expression of . may affect anti apoptosis
BCL2L2 PABPN1
through affecting the synergistic effect of and genes.
