Research progress of bacterial endotoxin detection methods
10.13200/j.cnki.cjb.003846
- VernacularTitle:细菌内毒素检测方法的研究进展
- Author:
ZHANG Yuan
- Publication Type:Journal Article
- Keywords:
Endotoxin;
Limulus amoebocyte;
Lipopolysaccharide(LPS);
Biosensor
- From:
Chinese Journal of Biologicals
2023;36(3):368-
- CountryChina
- Language:Chinese
-
Abstract:
As a powerful pyrogen substance,bacterial endotoxin in small amounts can cause many serious effects on human health and would cause fever,microcirculation disorders,endotoxemia,endotoxin shock,diffuse intravascular coagulation and even death.Therefore,it is very important to detect endotoxin in pharmaceutical products.In recent years,due to overfishing of horseshoe crab and environmental deterioration,the number of horseshoe crab in China is decreasing rapidly.It has been listed as the second-class protected animal in China,and the traditional endotoxin detection methods of limulus amoebocyte lysate will be replaced gradually.With the deepening of research,a series of rapid,sensitive and accurate methods for endotoxin detection have been developed.This paper reviews various endotoxin detection methods,focusing on their innovations such as recombinant factor C method and biosensor method,and elaborates their advantages,disadvantages and development trends with the hope that new detection technologies will be more widely developed and applied.
