Performance evaluation of colloidal gold test paper for Legionella pneumophila
10.13200/j.cnki.cjb.003835
- VernacularTitle:嗜肺军团菌胶体金检测试纸性能评估
- Author:
REN Meng
- Publication Type:Journal Article
- Keywords:
Legionella pneumophila(LP);
Colloidal gold immunochromatographic test paper;
Cross reactivity;
Sensitivity;
Hook effect
- From:
Chinese Journal of Biologicals
2023;36(3):306-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo prepare colloidal gold immunochromatographic test paper for rapid detection of Legionella pneumophila(LP)and test its performance to ensure that it meets the national clinical diagnostic standards.MethodsLP colloidal gold immunochromatographic test paper was prepared based on double antibody sandwich ELISA,and tested for the cross reactivity,anti-interference,sensitivity,hook effect,stability and other aspects.ResultsLP colloidal gold immunochromatography test paper showed no cross reaction with 22 common pathogens in respiratory tract such as Moraxella catarrhalis,and was not affected by internal and external interferences in respiratory tract;The minimum detection limit for LP was 2.00 × 105cfu/mL,with good sensitivity and no hook effect;Under the conditions of accelerated aging at 45 ℃,simulated high temperature transportation and frozen transportation,the repeatability and stability of test paper were not affected,and the stability was good in the same batch and between different batches.ConclusionThe prepared LP colloidal gold immunochromatographic test paper realized rapid detection of LP,which was simple to operate and had good application prospect and popularization value.
