Effect of Yangqing Chenfei Formula on epithelial-mesenchymal transition in silicon dioxide-induced silicosis rats
- Author:
WANG Xiang cheng LI Jian sheng TIAN Yan ge
- Publication Type:Journal Article
- Keywords:
Yangqing Chenfei Formula； ； ； ； ； Silicosis Epithelial interstitial transformation Pulmonary fibrosis Collagen Rat
- From:
China Occupational Medicine
2022;49(06):601-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective Yangqing Chenfei Formula -
To investigate the effect of (YCF) on epithelial mesenchymal transition (EMT)
Methods
in lung tissues of silicosis model rats. Specific pathogen free adult male SD rats were randomly divided into control
group, model group, tetrandrine group and YCF group, with eight rats in each group. The rats in the model group, tetrandrine
group and YCF group were intratracheally injected with 1.00 mL of silica suspension with a mass concentration of 50.0 g/L, and
the rats in the control group were given an equal volume of 0.9% sodium chloride solution. On the 15th day after modeling, the
tetrandrine group was given tetrandrine at a dose of 27.0 mg/kg body weight, the YCF group was given YCF with a dose of 8.91 g/kg
body weight, while both the control group and model group were given 2.00 mL 0.9% sodium chloride solution. Gavage wasperformed twice a day in the morning and evening for 14 days. On day 29 of the experiment, after evaluating the tidal volume,
-
functional residual volume (FRC) and vital capacity of rats in each group, lung tissues were collected, and hematoxylin eosin
staining and Masson staining were performed to examine the histopathological changes, and the fibrosis score was evaluated.
- -
Hydroxyproline level was detected by colorimetry. The expression of type Ⅰ collagen (COL Ⅰ), type Ⅲ collagen (COL Ⅲ),
- - - - - -
E cadherin (E Cad), N cadherin (N Cad) and α smooth muscle actin (α SMA) protein was detected by immunohistochemistry.
-
The expression of epithelial cell adhesion molecule (EpCAM) and fibroblast specific protein 1 (FSP 1) was detected by
Results
immunofluorescence. The lung structure was intact and the alveolar structure was normal in the control group. The
alveolar structure was destroyed, the alveolar wall was thickened, and cellular nodules were observed/n the model group. The
lung tissue lesions of rats in the tetrandrine group and YCF group were reduced compared with that in the model group, and there
was no difference in the degree of lesions between the two groups. The tidal volume, FRC and vital capacity of rats in model
P< - P<
group decreased (all 0.05), the relative expression of E Cad protein in lung tissue decreased ( 0.05), the fibrosis score and
- - - -
the level of hydroxyproline, the protein relative expression of COL Ⅰ, COL Ⅲ, N Cad and α SMA in lung tissue increased (all
P< -
0.05), while the fluorescence intensity of EpCAM protein decreased, and that of FSP 1 protein increased compared with the
P<
control group. The tidal volume, FRC and vital capacity of rats in tetrandrine and YCF groups increased (all 0.05), the fibrosis
- - -
score and the level of hydroxyproline, the protein relative expression of COL Ⅰ, N Cad and α SMA in lung tissue decreased (all
P< - P<
0.05), the relative expression of E Cad protein in lung tissues increased ( 0.05), while the EpCAM protein fluorescence
-
intensity increased and FSP 1 protein fluorescence intensity decreased compared with the model group. The relative expression
- P< Conclusion
of N Cad protein in lung tissues of YCF group was lower than that of the tetrandrine group ( 0.05). YCF can
-
improve the lung function, alleviate collagen deposition in lung tissues, and inhibit the epithelial mesenchymal transition in
silicosis model rats, and then attenuates the progression of silicotic fibrosis.
