Comparative analysis of neutralizing antibody titer and specific IgG concentration in sera immunized with mumps attenuated live vaccine(F-genotype)
10.13200/j.cnki.cjb.003851
- VernacularTitle:腮腺炎减毒活疫苗（F-基因型）免疫血清中和抗体效价与特异性IgG浓度的比较分析
- Author:
ZHAO Heng
- Publication Type:Journal Article
- From:
Chinese Journal of Biologicals
2023;36(3):263-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare and analyze neutralizing antibody titer and specific IgG concentration in the sera immunized with F-genotype mumps attenuated live vaccine,so as to evaluate the possibility of using them as mutual verification indexes.Methods A total of 194 serum samples were randomly selected,including 98 in vaccine group inoculated with F-genotype mumps attenuated live vaccine(49 before immunization and 49 after immunization)and 96 in placebo group(48 before immunization and 48 after immunization),which were detected for the concentration of mumps virus specific IgG and the titer of neutralizing antibody by ELISA and neutralization test respectively,and the detection results were compared.With neutralizing antibody titer as ordinate and IgG concentration as abscissa,the scatter plot was drawn,and the correlation between them was analyzed by logistic regression.With neutralizing antibody titer as standard,the specific IgG concentration of each group was analyzed by ROC curve,which was evaluated to judge the reliability of neutralizing antibody positive conversion.Results There was no significant difference in the concentration of specific IgG(t =-0.977,P > 0.05)and the titer of neutralizing antibody(Z =-1.405,P > 0.05)in the serum of the placebo group before and after immunization.However,in the vaccine group,the concentration of specific IgG(t =-9.959,P < 0.001)and the titer of neutralizing antibody(Z =-5.696,P < 0.001)in the serum after immunization were both significantly higher than those before immunization;The positive conversion rates of specific IgG before and after immunization in the placebo group and before immunization in the vaccine group were significantly higher than those of neutralizing antibody(χ2= 27.927,29.777 and 17.563respectively,each P < 0.001),while no significant difference was observed between the positive conversion rates of the two after immunization in the vaccine group(χ2= 27.927,29.777 and 17.563respectively,each P < 0.001),while no significant difference was observed between the positive conversion rates of the two after immunization in the vaccine group(χ2= 2.346,P > 0.05).There was a significant correlation between specific IgG concentration and neutralizing antibody titer in each group(r = 0.321 0 ～ 0.620 1,each P < 0.05).After immunization,the area under ROC curve was the largest(0.961),and the specificity(1.00)and sensitivity(0.93)were higher in the vaccine group.Conclusion The neutralizing antibody titer and specific IgG concentration in the serum immunized with mumps attenuated live vaccine(F-genotype)showed a good correlation,which might be used as mutual verification indicators.
