The chemical and pharmacological study of the new drug "Calvacin"
- VernacularTitle:Калвацин шинэ эмийн хими-фармакологийн судалгааны дүн
- Author:
Dejidmaa B
1
;
Nyamdemberel TS
;
Chimedragchaa CH
;
Dagvatseren B
;
Ganbold D
Author Information
1. Traditional Medical Science Technology and Production Corporation
- Publication Type:Journal Article
- Keywords:
burn;
calvacin;
experimental animal;
wound healing time;
- From:Mongolian Medical Sciences
2011;158(4):74-78
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background: Wound healing mechanisms still unclear. Wounds are physical injuries that result in an opening or breaking of the skin. Proper healing of wounds is essential for the restoration of disrupted anatomical continuity and disturbed functional status of the skin. This is a product of the integrated response of several cell types to injury. Wound healing is a complex process that results in the contraction and closure of the wound and restoration of a functional barrier.Goal: The purpose of this study was to determine burn wound healing activity of Calvacin. Materials and Methods:We used in study as colored reaction thin layer chromatography (TLC) and UV-spectrophotometer methods to determine active compounds in the three component drug "Calvacin".In research were inducted 20 healthy white mice and 40 rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Production Corporation and Institute of Veterinary medicine of pathological laboratory. The study protocol was approved by the Ethics Committee of the Ministry of Health.Results:Results of the phytochemical investigations showed that contained in the new drug "Calvacin" determined the presence of bioactive substances such as flavonoids, saponin, tannin, curcuminoids, organic acids and minerals. Substance was absorbed 25.7% in 40 grade ethanol, 27.1% in 70 grade, 22.2% in 96 grade and 19.6% in sterile water. We was established quality quantities of Calvacin in the drug amount summary flavonoids 1% and determined by UV spectrophotometer method. Proceeding from the absorption maximum of the substances analyzed the wave length of detection was determined as 370nm. In the result of mineral study of the "Calvacin", Ca^2.3%, P-6.17%, K-5.58%Burn wound healing time is control group 35±0.1 days, on 26±0.1 day animals of Calvacin group, but animals of Vishinski's ointment group 33±0.1 (p<0.05).Conclusions:1. The phytochemical investigations contained in the "Calvacin" drug determined the presence of bioactive substances such as flavonoids, saponin, tannin, curcuminoids, organic acids and minerals.2. Calvacin preparation has less toxic and significantly stimulate experimentally induced wound contract.
