The total protein and some characterization of allergenic proteins in the body of blatella germanica cockroach
- VernacularTitle:Blatella germanica жоомны биеийн нийт уураг, харшил төрүүлэх зарим шинж чанар
- Author:
Narantsetseg L
1
;
Oyuntsatsral B
;
Javzandolgor N
;
Myagmarsuren D
;
Munkhbayarlakh S
Author Information
1. School of Biomedicine, Health Sciences University of Mongolia
- Publication Type:Journal Article
- Keywords:
cockroach;
cockroach allergen;
total protein;
SDS-PAGE;
molecular weight;
asthma;
- From:Mongolian Medical Sciences
2012;161(3):17-19
- CountryMongolia
-
Abstract:
Introduction: Prevalence of asthma is increasing year by year, especially among children and exposure to high levels of indoor allergens is a very important factor [1]. Cockroaches are an important cause of asthma in many other regions of the world, including Taiwan, Thailand and Singapore in the Pacific Rim, Costa Rica and Puerto Rico in Centrel America, India, South Africa and more recently, Europe [2]. Goal: The aim of this study was determined total protein amounts allergenic proteins and protein bands of сockroach.Material and Methods: The сockroachs were collected in Ulaanbaatar. The allergenic protein components of the сockroach was purified by the method of Hames Richmond. The total protein of extracts was measured by the Bradford method and the protein components of cockroach were determined by the SDS-PAGE.Results: Among the 4000 known species of cockroaches, only 5 commonly inhabit homes and have the potential to contribute to indoor allergens. These include the American (periplaneta americana), German (Blattella germanica), Oriental (Blatta orientals), Smokey brown (Periplaneta fuliginosa), and brownbanded (Supella longipalpis) varieties [3]. We were defined 2,25mg/ml protein amounts (w/v) in extracts of the purified and lyophilized protein of the сockroach. We were used a standard marker 195,7; 104,0; 59,8; 41,6; 27,8; 21,1; 15,2; 6,5kd molecular weight proteins on the 13% separation gel of SDS-PAGE. On column determined protein bands with 82,3; 59,9; 55,2; 44,0; 41,6; 34,4, 22,7, 17,1 kd molecular weights.Conclusions: The сockroach was included 8 allergenic protein components between ranges of 17,1-82,3 kd molecular weights were determined in the extracts of the body Blatella germanica.
