Detection of human rhinoviruses in patients with acute respiratory infections in 2008-2012 in Mongolia
- VernacularTitle:Монгол улсад 2008-2012 онд бүртгэгдсэн амьсгалын замын өвчтэй хүмүүсээс хүний риновирүс илрүүлсэн дүн
- Author:
Tsatsral S
1
;
Maitsetseg CH
;
Nymadawaa P
Author Information
1. National Center of Communicable Diseases
- Publication Type:Journal Article
- Keywords:
rhinovirus;
multiplex PCR;
genotype;
- From:Mongolian Medical Sciences
2013;165(3):16-20
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background: Human rhinoviruses (HRVs) consist of approximately 150 types that cause a wide range of clinical outcomes, including asymptomatic infections, common cold, sinusitis, bronchiolitis, asthma and pneumonia. Rhinovirus illnesses are common inall age groups, they occur throughout the year and they are present world-wide. The prevalence of this virus has not been studied so far in Mongolia. Method: Nasopharyngeal swabs were collected between November 2008 to December 2012 from 2218 persons with acute respiratory tract infections. HRVs in the clinical samples were detected by multiplex real-time PCR with the commercial kit “Respiratory pathogen 21, FTD”, Fast Track Diagnostics, Luxembourg). We have used three sets of primers to detect and sequence all HRV positive samples with.Results: HRVs were detected in 257(23.4%) patients. These patients were ranged in age from 0 month to 63 years old, HRVs detected from all ages. Co-infections with other respiratory viruses were detected in 59(23%) of the HRV positive patients. HRV was detected whole year around and the peak seasonal prevalence was in early fall. We have genotyped 34(14.2%) HRV positive samples randomly selected from 240 samples. Out of 34 tested for genotyping rhinoviruses, 22(65%) tested positive for HRV-A, 9(26%) for HRV-C, only 3(9%) for HRV-B.
