Activity of the hydroquinone its based TLC/HPLC analysis
- VernacularTitle:Нимгэн үет хроматограф болон өндөр даралтат шингэний хроматографийн аргаар гидрохиноны идэвхийг тодорхойлсон дүн
- Author:
Byambasuren D
1
Author Information
1. School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Arbutin;
Glycosylation;
Glycosyltransferse;
Hydroquinone;
HPLC;
TLC;
- From:Mongolian Medical Sciences
2014;169(3):14-17
- CountryMongolia
- Language:Mongolian
-
Abstract:
BackgroundHydroquinone (1,4 di-hydroxy benzene) is an aromatic organic compound with diverse biologicalfunctions that are commonly used as pigment or cosmetic additives. Its chemical structure has twohydroxyl groups bonded to a benzene ring in a para position. In the nature hydroquinone have a primaryreagent in the defensive glands of bombardier beetles, along with hydrogen peroxide, which collects in areservoir [1]. Hydroquinone glycoside form is 4-hydroxyphenyl-α-D-glucopyranoside (α- Arbutin). Arbutinis a potent inhibitor of melanin synthesis and has been reported to possess inhibitory activity on lipidperoxidation and is used in the cosmetic industry for its antibacterial and skin lightening effects [9]. It isfaster and more effictive another commonly used skin lightener, even at use very low concentrations.Material and MethodsThe in vitro glycosylation reaction was carried out as described in materials and methods. DNA wasextracted than E.coli BL 21 and E.coli JM 109 hosts were used for expression of proteins. The purifiedprotein was then analyzed by 12% SDS-PAGE than used for enzymatic recycle system. Hydroquinonereaction mixture was incubated at 37ºC for 8 and 24 hour than quenched by heating in boiling waterfor 10 min. The reactions products was first analyzed by TLC followed by HPLC analysis.ResultsThis work substrate hydroquinone for the enhancement of enzymatic recycling system glycosylation. TLCand HPLC analyses of the products were carried out to the recycled system worked and glycosylationproduct.ConclusionIn this study, hydroxyl groups of the para position of hydroquinone involved for glycosylation to comparewith standards were analysed by TLC and HPLC. When the compared 8 hour reactions obtained theapparent glycosylation and stability of the recycling system.
