Development and Validation of an UV Spectrophotometric Method of Quantification of Fluconazole Reference
- VernacularTitle: Стандарт флуконазолыг хят- ны спектрофотометрийн аргаар тодорхойлж баталгаажуулах нь
- Author:
Gereltuya DORJ
;
Sarnaizul ERDENEBAATAR
;
Zoljargal SAMBUU
;
Nomin-Erdene TSOLMON
;
School of Pharmacy and Biomedicine, MNUMS, United family, Intermed hospital,National second hospita
- Publication Type:Journal article
- Keywords:
Fluconazole;
method development;
validation;
spectrophotometry
- From:
Mongolian Pharmacy and Pharmacology
2016;9(2):11-15
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction: A UV-spectrophotometric method has been developed for the quantitative estimationof fluconazole. The present study describes development and validation of UV-spectroscopicmethod for estimation of fluconazole reference substance and validation of detection method.Method: During development of analytical method HCl: water (6 different concentrations).The detection length (cmax) was found to be 260nm. Calibration curves were prepared. The proposedmethod obeys Beer’s law in the range of 5-50mkg/ml. Absorption maxima was determinedwith 10 mkg/ml by scanning in the range of 200-400nm.Results: Percent recovery studies are in the range of 95,12%-101.89%. The method was validatedin terms of specificity, linearity, accuracy, range, precision, repeatability, robustness, stabilityof analytical solution. Results of analysis were validated statistically and by specificity studies.From that it was observed that there is no interference of blank, excipients during the estimationof reference substance in formulation.Conclusion: This shows the adaptability of the method for routine estimation of fluconazolereference. Keywords: Fluconazole, method development, validation, spectrophotometry
