"Disease-syndrome-therapy" Spontaneous Abortion Models and Application Trends of Single-cell Multimodal Omics： A Review
10.13422/j.cnki.syfjx.20230519
- VernacularTitle:“病-证-治”结合自然流产模型研究进展及在单细胞多模态组学的应用趋势
- Author:
Linwen DENG
1
;
Ying TANG
1
;
Yi YANG
1
;
Shuguang ZHANG
1
;
Weijun DING
2
;
Hang ZHOU
2
Author Information
1. Hospital of Chengdu University of Traditional Chinese Medicine（TCM）， Chengdu 610072， China
2. School of Basic Medicine， Chengdu University of TCM， Chengdu 611137， China
- Publication Type:Journal Article
- Keywords:
spontaneous abortion;
combination of diseases and syndromes;
animal models;
research progress;
development trend
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(14):191-203
- CountryChina
- Language:Chinese
-
Abstract:
The incidence of spontaneous abortion （SAB） has been increasing year by year， and its etiology is complex， with limited treatment options， which poses a serious threat to social stability. The "disease-syndrome-therapy" research model can significantly improve the clinical efficacy of traditional Chinese medicine （TCM） for preventing miscarriage， but there has always been a lack of key and recognized diagnostic and treatment evaluation markers， which need to be further explored to establish a scientific and unified evaluation standard system. It is proposed to collect existing "disease-syndrome-therapy" SAB animal models， transplant and improve the model evaluation indicators， evaluate the degree of match between SAB animal models and the clinical characteristics of TCM and Western medicine diseases and syndromes， and compare the advantages and disadvantages of different SAB animal models in terms of construction methods， target selection， and evaluation indicators. In addition， the frontiers of TCM experimental research will be explored. In view of the current status and related bottlenecks of molecular biomarkers research on SAB TCM animal models， a single-cell multimodal omics research strategy will be proposed to break through the related evaluation defects of the "disease-syndrome-therapy" SAB and analyze the differences in various cell types， cell subpopulations， spatiotemporal trajectories， and gene expression in the mother-fetal interface tissue at the single-cell level. This will provide accurate guidance and model animal platform support for the in-depth study of disease-syndrome models， Zang-fu biology， and novel targeted drugs. It will also provide a basis for establishing a stable and repeatable "disease-syndrome-therapy" SAB animal model and evaluation indicator system， which is beneficial for the long-term development of TCM reproductive animal model research.
