iTRAQ-based Proteomic Analysis of Serum Differential Proteins from Henoch-Schönlein Purpura Patients Before and After Qishun Baolier Treatment
10.13422/j.cnki.syfjx.20230347
- VernacularTitle:基于iTRAQ技术筛选齐顺保利尔治疗过敏性紫癜前后的血清差异蛋白
- Author:
Nashun WURITU
1
;
Batu BUREN
1
;
Qiqige NAREN
1
;
Guniang JIU
1
;
Duleng EERDUN
1
;
Shuanglian WANG
1
;
Cuiqin GONG
1
;
Gaowa HASHEN
1
;
Huizhi JIN
1
;
Haizi TING
1
;
Wurihan BAI
1
Author Information
1. Affiliated Hospital of Inner Mongolia Minzu University，Tongliao 028000，China
- Publication Type:Journal Article
- Keywords:
Henoch-Schönlein purpura（HSP）;
immune response;
Qishun Baolier;
isobaric tags for relative and absolute quantification（iTRAQ）;
proteomics;
biomarkers;
serum proteins
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(14):105-113
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveHenoch-Schönlein purpura（HSP） is one of the dominant diseases in Mongolian medicine. Qishun Baolier（QSBLE）， as the main prescription for the treatment of HSP， has significant clinical effect， but its mechanism is not yet clear. Baed on this， this study is intended to screen the differentially expressed proteins before and after treatment， and preliminarily explore the molecular mechanism of QSBLE in the treatment of HSP. MethodTaking oneself as the control， 30 HSP patients aged 6-45 years were collected， and QSBLE was taken orally at 12：00 and 24：00， respectively. The dose was adjusted according to age and the course of treatment was one week. The distribution of proteinuria， hematuria and skin purpura of all patients were determined before and after treatment. The serum samples of 10 patients with clinically significant remission after QSBLE treatment were randomly selected for proteomics. Isobaric tags for relative and absolute quantification（iTRAQ） combined with liquid chromatography tandem mass spectrometry（LC-MS/MS） was used to analyze the proteins in serum of HSP patients before and after treatment， and differential proteins were analyzed bioinformatically and the protein-protein interaction（PPI） networks were constructed. ResultA total of 378 proteins were identified from serum， including 18 differentially expressed proteins， of which 15 proteins were up-regulated and 3 proteins were down regulated. Bioinformatics showed that the differential proteins were mainly involved in biological processes such as immune response， immunoglobulin production， phagocytosis， adaptive immune response before and after treatment. Biological processes， pathways and proteins were used to construct the PPI network， the proteins represented by immunoglobulin heavy constant γ1（IGHG1）， immunoglobulin λ-chain 7-43（IGLV7-43）， gelsolin（GSN） and 60 kDa heat shock protein（HSPD1） were involved in biological processes and related pathways such as adaptive immune response， immunoglobulin production， leukocyte-mediated immunity， regulation of stress response， regulation of immune system processes， regulation of trauma response， and these proteins were at the center of the PPI network. ConclusionQSBLE may play a role in the treatment of HSP by regulating the expression of IGHG1， IGLV7-43， GSN， HSPD1 and other key proteins to affect immune-related biological processes.
