Mechanisms and Effect of Ginkgolide B on the Differentiation of Neuron Stem Cells
- VernacularTitle:银杏内酯B对神经干细胞分化的影响及其机制研究
- Author:
Yonghong WANG
;
Xue LUO
;
Yongjiang SHI
;
Hongliang LIU
;
Zhongxiang YAO
- Publication Type:Journal Article
- Keywords:
Ginkgolide B, neuron stem cells, differentiation, suppressor of cytokine signaling-2 (SOCS2), inhibitor of DNA binding-2 (Id2)
- From:
Chinese Journal of Rehabilitation Theory and Practice
2007;13(8):701-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Ginkgolide B of various consistency on the differentiation of neuron stem cells (NSCs).MethodsNSCs were cultured in differentiation medium containing Ginkgolide B of various consistency for 3 and 7 days, the neurites length and cell body area were measured by inverted phase-contrast micrograph, then neurofilament-200 (NF-200), glial fibrillary acidic protein (GFAP), adenomatus polyposis coli (CC-1) expression were detected and counted by fluorescence microscope. The suppressor of cytokine signaling-2 (SOCS2), inhibitor of DNA binding-2 (Id2) were alsoimmunostained. The percentage of positive cells were counted respectively.ResultsThe neurites length and cell body area in Ginkgolide B groups were obviously larger than that in the control group. The percentage of NF, GFAP positive cells in Ginkgolide B groups increased with dosage increasing of Ginkgolide B. Compared with the normal control group, the percentage of SOCS2 positive cells increased significantly ( P<0.01) and the percentage of Id2 positive cells decreased significantly ( P<0.01) in Ginkgolide B groups.ConclusionGinkgolide B can promote NSCs to differentiate into neuron and astrocyte, the percentage of astrocyte is increased with a dosage-dependent relationship with Ginkgolide B.
