Gene mutation associated with drug resistance in M.tuberculosis strains isolated from national TB prevalence survey in Mongolia
- VernacularTitle:Монгол улсын хүн амын дундах сүрьеэгийн тархалтын судалгаагаар ялгасан M.tuberculosis омгийн эмэнд тэсвэртэй генийн мутацийг тодорхойлсон дүн
- Author:
Tsetsegtuya B
1
;
Baasansuren E
1
;
Oyuntuya T
1
;
Tserelmaa B
1
;
Gundsuren Sh
1
;
Tsolmon B
1
;
Naranbat N
2
;
Tsatsralt-Od B
1
;
Buyankhishig B
1
;
Naranzul D
1
Author Information
1. National Center for Communicable Disease
2. Mongolian Anti-tuberculosis Coalition
- Publication Type:Journal Article
- Keywords:
drug resistance;
inhA;
isolates;
isoniazid;
gene mutation;
katG rifampicin
- From:Mongolian Medical Sciences
2019;187(1):3-10
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:According to the First National Tuberculosis (TB) Prevalence Survey in Mongolia the prevalence of
bacteriologically-confirmed pulmonary TB among adults was 559.6 (95% CI: 454.5–664.7) per 100000
population in 2014–2015. This was three times as high as previously estimated. Nationwide anti-tuberculosis (TB) drug resistance survey was conducted in 1999 and 2007 in Mongolia. Share of multidrug resistant TB (MDR-TB) cases among newly notified TB cases increased from 1.0% in 1999 to 1.4% in 2007. Accordingly, we aimed to perform drug susceptibility test on strains isolated from TB Prevalence Survey and to determine the prevalence of drug resistant TB.
Material and Methods:All 242 MTB strains isolated from the survey TB cases were tested GenoTypeMTBDRplus test and conventional 1st line DST on solid medium.
Result:Conventional DST and GenoTypeMTBDRplus tests done for 93.8% (227/242) of them and 6.2% (15/242) were tested by GenoTypeMTBDRplus only. A 61.6% (95%CI 55.3-67.4) of all cases were susceptible to first line anti-TB drugs, any drug resistance and MDR-TBdetected as 38.4% (95% CI 32.5-44.7)and 9.5% (95% CI 6.4-13.9), respectively. Prevalence of MDR-TB was7.8% (95% CI 4.9-12.4) among new and 17.9% (95% CI 9.0-32.7) among previously treated cases. The 64 strains were identified as a resistant to isoniazid, 32.8% (42/64) and 65.6% (21/64) were katG, and inhAmutation, respectively. One isolate (1.6%) was mutations in both the inhAand katGgenes.The predominant mutations detected in therpoB were S531L (91.3%) among rifampicin resistant isolates and the mutation in inhAwas C–15T (100%) and katG mutation was S315T1 (100%) among isoniazid-resistant isolates.
Conclusion:Prevalence of cases with DR-TB is high among prevalent TB cases, especially prevalence of MDR-TB among new cases. In comparison to previous studies, DR-TB cases seem to be increased. Rifampicin resistant strains have a mutation of the rpoBand resistance to isoniazid is predominantly associated with the inhA mutation.
- Full text:2019-187(1);3-10.pdf
